LOCUS       sequence-123784-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: bicistronic; 
            FRT-galK-FRT in 2nd mChO intron;use C-term after STOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU12
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    531..564
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     573..589
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     573..589
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 caatactcag gggatgtcag cgagcattgg gtaccgagcg tcggatccac gtaggtaagg
       61 cttnanagtg ctgctggaat tcgcccattt tacttataca attcatccat gccacctgtc
      121 gagtgccgcc cttcagctct ttcatattgc tcaacgatag tgtaatcctc gttgtgggag
      181 gtgatatcca gtttaatatt aacattgtac gctcctggca gctgaactgg ttttttggct
      241 ttgtaagttg tcttcacctg aaaatttaaa tcagattagt tagttagtaa aatcatgttt
      301 aaacttacct cagcatcgta atgtcctcca tctttcaatt taagacgttg tttaatctct
      361 cccttaagag caccgtcttc agggtacatt ctttcacttg aagcttccca tcccatagtc
      421 ttcttctgca taacaggtcc atccgagggg aaattcgttc ctcgaagttt gactttataa
      481 ataaattctc catcttggag gctgaaaatt taaatatgta tggttagtta gaagttccta
      541 ttctctagaa agtataggaa cttcgcggcc gctctagaac tagtggatcc tcagcactgt
      601 cctgctcctt gtgatggttt acaaacgtaa aaagtctctt taatacctgt ttttgcttca
      661 tattgttcag cgacagcttg ctgtacggca ggcaccagct cttc
//
