LOCUS       sequence-123793-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding tRNA-SupD for serine amber suppression. *Update: 
            Please use the updated version, Plasmid 198598: SupD v2, which is 
            more stable..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: SupD
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        728..805
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 tattgataat cctgatatga gtaaattgca gtttcatttg atgctcgatg agtttttcta
       61 agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
      121 ttccgcgcac atttccccga aaagtgccac cgcggccgcg cttctttgag cgaacgatca
      181 aaaataagtg gcgccccatc aaaaaaatat tctcaacata aaaaactttg tgtaatactt
      241 gtaacgctga attcggagag atgccggagc ggctgaacgg accggtctct aaaaccggag
      301 taggggcaac tctaccgggg gttcaaatcc ccctctctcc gccaggatcc tctagagtcg
      361 acctgcagat ccttagcgaa agctaaggat tttttttgcc gcggcgcttc tttgagcgaa
      421 cgatcaaaaa taagtggcgc cccatcaaaa aaatattctc aacataaaaa actttgtgta
      481 atacttgtaa cgctgaattc ggagagatgc cggagcggct gaacggaccg gtctctaaaa
      541 ccggagtagg ggcaactcta ccgggggttc aaatccccct ctctccgcca ggatcctcta
      601 gagtcgacct gcagatcctt agcgaaagct aaggattttt tttgcggccg ctttcaccgt
      661 catcaccgaa acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag cggcatgcat
      721 ttacgttgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga
      781 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
      841 tgc
//
