LOCUS       sequence-123796-         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses dsRed and shRNA to knock down adra2a receptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Zhang Z, Ferretti V, Guntan I, Moro A, Steinberg EA, Ye Z, Zecharia 
            AY, Yu X, Vyssotski AL, Brickley SG, Yustos R, Pillidge ZE, Harding 
            EC, Wisden W, Franks NP
  TITLE     Neuronal ensembles sufficient for recovery sleep and the sedative 
            actions of alpha2 adrenergic agonists.
  JOURNAL   Nat Neurosci. 2015 Apr;18(4):553-61. doi: 10.1038/nn.3957. Epub 2015
            Feb 23.
  PUBMED    25706476
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3957"; 
            journalName: "Nat Neurosci"; date: "2015-04"; volume: "18"; issue: 
            "4"; pages: "553-61"
COMMENT     Sequence Label: AAV-actin prom-dsRed-adra2a.1227.shRNA
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           24..145
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           173..187
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           215..341
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        495..597
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gcttcggcag cacatatact atgttgaatg aggcttcagt actttacaga atcgttgcct
       61 gcacatcttg gaaacacttg ctgggattac ttcttcaggt taacccaaca gaaggctcga
      121 gaaggtatat tgctgttgac agtgagcgag caacgtgctg gttattatcg tagtgaagcc
      181 acagatgtac gataataacc agcacgttgc ctgcctactg cctcggaatt caaggggcta
      241 ctttaggagc aattatcttg tttactaaaa ctgaatacct tgctatctct ttgatacatt
      301 tttacaaagc tgaattaaaa tggtataaat taaatcactt ttttcaattg gaagactaat
      361 gcgtttaaac acgcggcgac gcgttcgacc gaataaatac ctgtgacgga agatcacttc
      421 gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg
      481 cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
      541 tcactaccgg gcgtattttt tgagttgtcg agattttcag gagctaagga agctaaaatg
//
