LOCUS       sequence-123804-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing nuclease null SaCas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: AAV_NLS-dSaCas9-NLS-VPR
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctgatcttg ctgtcgctgc tgctcaggta ctggaatggg gtccggttgc ccttcttgct
       61 ggcttcttcc tgcttcacga gcaccttgtt gttgaagctg ttgtcgaagg acacgcttct
      121 ggggatgatg tggtccacct catagttgaa ggggttgttc agcagatctt ccagagggat
      181 ggcttccagg ctgtacaggc acttgccttc ctgcatgtcg tgcagcttga tcttctcgat
      241 caggtacttg gcgttctctt tgccggtggt ccggatgatt tcctcgatcc gctcgttggt
      301 ctgccggttc cgcttctgca tctcgttgat cattttctgg gcgtccttgg agttcttctc
      361 gcgggccagc tcgataatga tgtcgttggg caggccgtac ttcttgatga tggcgttgat
      421 cactttgatg ctctggatga agcttctctt cacgacgggg ctcaggatga agtcgtccac
      481 cagggtggtg gggatctctt tctgctggga caggtccacc ttcttgggca ccagcttcag
      541 ccggttgaag atagcgatct ggttgtcgtt ggtgtgccac agctcgtcca ggatcaggtt
      601 gatggccttc aggctcaggt tgtgggtgcc ggtatagccc ttcagattag agatctgctc
      661 gatctcttcc tgggtcagct cggagttcag attggtcagt tcttcctgga
//
