LOCUS       sequence-123807-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Phosphoserine orthogonal translation system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: SepOTS-iota
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        703..780
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(800..819)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tatggtattg ataatcctga tatgagtaaa ttgcagtttc atttgatgct cgatgagttt
       61 ttctaagaat taattcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      121 aggggttccg cgcacatttc cccgaaaagt gccaccgcgg ccgcgcttct ttgagcgaac
      181 gatcaaaaat aagtggcgcc ccatcaaaaa aatattctca acataaaaaa ctttgtgtaa
      241 tacttgtaac gctgaattcg ccggggtagt ctaggggtta ggcagcggac tctaaatccg
      301 ccttacgtgg gttcaaatcc cacccccggc tccaggatcc tctagagtcg acctgcagat
      361 ccttagcgaa agctaaggat tttttttgcc gcggcgcttc tttgagcgaa cgatcaaaaa
      421 taagtggcgc cccatcaaaa aaatattctc aacataaaaa actttgtgta atacttgtaa
      481 cgctgaattc gccggggtag tctaggggtt aggcagcgga ctctaaatcc gccttacgtg
      541 ggttcaaatc ccacccccgg ctccaggatc ctctagagtc gacctgcaga tccttagcga
      601 aagctaagga ttttttttgc ggccgctttc accgtcatca ccgaaacgcg cgaggcagca
      661 gatcaattcg cgcgcgaagg cgaagcggca tgcatttacg ttgacaccat cgaatggtgc
      721 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
      781 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctt
//
