LOCUS       sequence-123810-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the UCR1 region of PDE4D3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Wang L, Burmeister BT, Johnson KR, Baillie GS, Karginov AV, Skidgel 
            RA, O'Bryan JP, Carnegie GK
  TITLE     UCR1C is a novel activator of phosphodiesterase 4 (PDE4) long 
            isoforms and attenuates cardiomyocyte hypertrophy.
  JOURNAL   Cell Signal. 2015 May;27(5):908-22. doi: 
            10.1016/j.cellsig.2015.02.003. Epub 2015 Feb 12.
  PUBMED    25683917
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cellsig.2015.02.003"; journalName: "Cell Signal"; date: 
            "2015-05"; volume: "27"; issue: "5"; pages: "908-22"
COMMENT     Sequence Label: FLAG-Cerulean-UCR1
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..47
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(51..53,54..56,57..767)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(96..117)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(357..376)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     704..725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accgtcagaa ttgatctacc atggactaca aagacgatga cgacaagctt atggtgagga
       61 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      241 ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
      301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      481 acaacgccat cagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
      541 ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      661 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      721 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggcg aattcctttg
      781 atgtggacaa tggcacatct gcgggacgga gtcccttgga tcccatgacc agcccaggat
      841 ccgggctaat tctccaagca a
//
