LOCUS       sequence-123814-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal amino acids of UCR1 region of PDE4D3 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Wang L, Burmeister BT, Johnson KR, Baillie GS, Karginov AV, Skidgel 
            RA, O'Bryan JP, Carnegie GK
  TITLE     UCR1C is a novel activator of phosphodiesterase 4 (PDE4) long 
            isoforms and attenuates cardiomyocyte hypertrophy.
  JOURNAL   Cell Signal. 2015 May;27(5):908-22. doi: 
            10.1016/j.cellsig.2015.02.003. Epub 2015 Feb 12.
  PUBMED    25683917
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cellsig.2015.02.003"; journalName: "Cell Signal"; date: 
            "2015-05"; volume: "27"; issue: "5"; pages: "908-22"
COMMENT     Sequence Label: FLAG-RFP-UCR1C
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..47
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             51..761
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    762..827
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 accgtcagaa ttgatctacc atggactaca aagacgatga cgacaagctt atggtgtcta
       61 agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga
      121 acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
      181 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
      241 ccagcttcat gtacggcagc agaaccttca tcaaccacac ccagggcatc cccgacttct
      301 ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
      361 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
      421 agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct
      481 gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctggaaggc agaagcgaca
      541 tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttcaagacc acatacagat
      601 ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac cacagactgg
      661 aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca
      721 gatactgcga cctccctagc aaactggggc acaaacttaa ttccggactc agatctcgag
      781 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc gg
//
