LOCUS       sequence-123821-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint frame selector +2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry-Frame +2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(262..337)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(358..606)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(416..435)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(586..606)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(633..649)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(665..687)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 cagtttaccg taatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
       61 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
      121 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
      181 cgtaattgat tactattaat aactagacaa taatcaatgt caacgcgtat atctggcccg
      241 tacatcggaa ttcgaaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac
      301 tagccttatt ttaacttgct atttctagct ctaaaaccgc tttttgggta ctggcccggt
      361 gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt
      421 aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta
      481 ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattcc
      541 aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag
      601 gccctcggat ccggccggcg cctagagaag gagtgagggc tggataaagg gaggatcgag
      661 gcggggtcga acgaggaggt tcaaggggga gagacggggc ggatggagga agaggaggcg
      721 gaggcttagg gtgtacaaag ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc
      781 aggtcacgat gtaggggacc tggtctgggt gtccatgcgg gccaggtgaa aagaccttga
      841 tcttaacctg
//
