LOCUS       sequence-123851-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fluorescent chitin reporter under Gal4 control in 
            Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Sobala LF, Wang Y, Adler PN
  TITLE     ChtVis-Tomato, a genetic reporter for in vivo visualization of 
            chitin deposition in Drosophila.
  JOURNAL   Development. 2015 Nov 15;142(22):3974-81. doi: 10.1242/dev.126987. 
            Epub 2015 Sep 22.
  PUBMED    26395478
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.126987"; journalName: "Development"; date: "2015-11-15-
            15"; volume: "142"; issue: "22"; pages: "3974-81"
COMMENT     Sequence Label: pWalium-ChtVis-Tomato
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    38..468
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     polyA_signal    490..611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(501..520)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     555..574
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(883..903)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggagctcgtt gttggttggc acaccacaaa
       61 tatactgttg ccgagcacaa ttgatcggct aaatggtatg gcaagaaaag gtatgcaata
      121 taataatctt ttattgggta tgcaacgaaa atttgtttcg tcaacgtatg caatattctt
      181 tattaaaaga gggtatgcaa tgtattttat taaaaacggg tatgcaatat aataatcttt
      241 tattgggtat gcaacgaaaa tttgtttcgt caaagtatgc aatatttttt attaaaagag
      301 ggtatgcaat gtattttatt aaaaacgggt atgcaataaa aaattatttg gtttctctaa
      361 aaagtatgca gcacttattt tttgataagg tatgcaacaa aattttactt tgccgaaaat
      421 atgcaatgtt tttgcgaata aattcaacgc acacttatta cgtggccaga gctctggaac
      481 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
      541 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      601 ataaacaagt taacaacaac aattccattc attttatgtt tcaggttcag ggggaggtgt
      661 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatctct
      721 agtcaaggca ctatacatca aatatttttc cataattttc ttgtatagca gtgcagcttt
      781 ttcctttgtg gtgtaaatag caaagcaagc aagagttcta ttactaaaca cagcatgact
      841 caaaaaactt agcaattctg aaggaaagtc cttggggtct tctacctttc tcttcttttt
      901 tggaggagta gaatgttgag agtcagcagt agcctcatca tcactagatg gcatttcttc
      961 tgagcaaaa
//
