LOCUS       sequence-123858-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SMASh tag fused to YFP at C terminus in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, 
            Lin MZ
  TITLE     Tunable and reversible drug control of protein production via a 
            self-excising degron.
  JOURNAL   Nat Chem Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869.
  PUBMED    26214256
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869."
COMMENT     Sequence Label: pAG426GPD-ccdB-YFP-yeastSMASh
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             104..355
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     292..313
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             416..439
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
       61 gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg
      121 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga
      181 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      241 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      301 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggacga
      361 aatggaagaa tgtagtaagc acttacctgg tgcaggctca tctggcgata tcatggacta
      421 caaggacgac gatgataagg gttcttctgg aacaggttca ggttcaggta caagtgcacc
      481 cattactgct tatgctcagc aaacaagagg cctattgggt tgtattatta ctagtttgac
      541 gggcagagac aaaaaccagg tagaaggtga agtgcagatc gtatccacgg ctactcagac
      601 attcttggct acttgtatta atggtgtgtg ttgggcagtt taccacggtg cgggaactag
      661 aactattgcc tctcctaagg gtccagtgat ccaaatgtat actaacgttg atcaagattt
      721 ggtcggctgg ccagctccac aagggtcaag aagcctaact ccttgcacct gcggttcttc
      781 agatctgtac ttagtgacac gtcatgcaga tgttatacca gttagacgtc gtgg
//
