LOCUS       sequence-123859-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SMASh tag fused to YFP at N terminus in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, 
            Lin MZ
  TITLE     Tunable and reversible drug control of protein production via a 
            self-excising degron.
  JOURNAL   Nat Chem Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869.
  PUBMED    26214256
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869."
COMMENT     Sequence Label: pCS6-SMASh-YFP
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             232..255
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggtccggaat tcggtgcaga gctcaagcga gttctcccgc agccgcagtc tctgggcctc
       61 tctagcttca gcggcgacga gcctgccaca ctcgctaagc tcctccggca ccgcacacct
      121 gccactgccg ctgcagccgc cggctctgct cccttccggc ttctgcctca gaggagttct
      181 tagcctgttc ggagccgcag caccgacgac cagatggagc ccgggcccat ggattacaag
      241 gatgacgacg ataagggctc ttccgggaca ggctccggat ccggcactag tgcgcccatc
      301 acggcgtacg cccagcagac gagaggcctc ctagggtgta taatcaccag cctgactggc
      361 cgggacaaaa accaagtgga gggtgaggtc cagatcgtgt caactgctac ccaaaccttc
      421 ctggcaacgt gcatcaatgg ggtatgctgg gcagtctacc acggggccgg aacgaggacc
      481 atcgcatcac ccaagggtcc tgtcatccag atgtatacca atgtggacca agaccttgtg
      541 ggctggcccg ctcctcaagg ttcccgctca ttgacaccct gtacctgcgg ctcctcggac
      601 ctttacctgg tcacgaggca cgccgatgtc attcccgtgc gccggcgagg tgatagcagg
      661 ggtagcctgc tttcgccccg gcccatttcc tacttgaaag gctcctcggg gggtccgctg
      721 ttgtgccccg cgggacacgc cgtgggccta ttcagggccg cggtgtgcac ccgtggagtg
      781 gctaaagcgg tggactttat ccctgtggag aacctagaga caaccatgag atccccggtg
      841 ttcacggaca actcctctcc accagcagtc accctgacgc accc
//
