LOCUS       sequence-123860-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SMASh tag fused to YFP at N terminus in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, 
            Lin MZ
  TITLE     Tunable and reversible drug control of protein production via a 
            self-excising degron.
  JOURNAL   Nat Chem Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869.
  PUBMED    26214256
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869."
COMMENT     Sequence Label: pCS6-SMASh-YFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..230
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
       61 aagcactgca ggccgtagcc cagggtggtc acgagggtgg gccagggcac gggcagcttg
      121 ccggtggtgc agatcagctt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      181 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      241 accccggtga acagctcctc gcccttgctc accataggcg cgcccgagcc cattgagcat
      301 ggcacgacat cttcgaactc ctggtagaga acctccctgt caggtataat actgcttccg
      361 ctactgcctg ccggcttccc ggacaagacg atcctgccca ctatgaccac gcagcctgtt
      421 gacaggcaat acgcggccag agcagccagg acgccgccaa cgagcaccca ggtgctcgtg
      481 acgacctcca ggtcggccga catgcatgtc atgatgtatt tggtatcgat tttggtgatt
      541 gggtgcgtca gggtgactgc tggtggagag gagttgtccg tgaacaccgg ggatctcatg
      601 gttgtctcta ggttctccac agggataaag tccaccgctt tagccactcc acgggtgcac
      661 accgcggccc tgaataggcc cacggcgtgt cccgcggggc acaacagcgg accccccgag
      721 gagcctttca agtaggaaat gggccggggc gaaagcaggc tacccctgct atcacctcgc
      781 cggcgcacgg gaatgacatc ggcgtgcctc gtgaccaggt aaaggtccga ggagccgcag
      841 gtacagggtg tcaatgagcg
//
