LOCUS       sequence-123865-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SMASh tag fused to YFP at C terminus in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, 
            Lin MZ
  TITLE     Tunable and reversible drug control of protein production via a 
            self-excising degron.
  JOURNAL   Nat Chem Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869.
  PUBMED    26214256
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869."
COMMENT     Sequence Label: pCS6-YFP-SMASh
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..353
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     290..311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             420..443
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
       61 tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
      121 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
      181 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      241 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      301 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagcccgggg
      361 atgagatgga agagtgctct cagcacttac ccggcgccgg cagtagtggc gatatcatgg
      421 attacaagga tgacgacgat aagggctctt ccgggacagg ctccggatcc ggcactagtg
      481 cgcccatcac ggcgtacgcc cagcagacga gaggcctcct agggtgtata atcaccagcc
      541 tgactggccg ggacaaaaac caagtggagg gtgaggtcca gatcgtgtca actgctaccc
      601 aaaccttcct ggcaacgtgc atcaatgggg tatgctgggc agtctaccac ggggccggaa
      661 cgaggaccat cgcatcaccc aagggtcctg tcatccagat gtataccaat gtggaccaag
      721 accttgtggg ctggcccgct cctcaaggtt cccgctcatt gacaccctgt acctgcggct
      781 cctcggacct ttacctggtc acgaggcacg ccgatgtcat tc
//
