LOCUS       sequence-123866-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SMASh tag fused to YFP at N terminus in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, 
            Lin MZ
  TITLE     Tunable and reversible drug control of protein production via a 
            self-excising degron.
  JOURNAL   Nat Chem Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869.
  PUBMED    26214256
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869."
COMMENT     Sequence Label: pAG426GPD-ccdB-yeastSMASh-YFP
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..80
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     64..80
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 attctacttt tatagttagt ctttttttta gttttaaaac accagaactt agtttcgacg
       61 gattctagaa ctagtggatc catggcgccc atcacggcgt acgcccagca gacgagaggc
      121 ctcctagggt gtataatcac cagcctgact ggccgggaca aaaaccaagt ggagggtgag
      181 gtccagatcg tgtcaactgc tacccaaacc ttcctggcaa cgtgcatcaa tggggtatgc
      241 tgggcagtct accacggggc cggaacgagg accatcgcat cacccaaggg tcctgtcatc
      301 cagatgtata ccaatgtgga ccaagacctt gtgggctggc ccgctcctca aggttcccgc
      361 tcattgacac cctgtacctg cggctcctcg gacctttacc tggtcacgag gcacgccgat
      421 gtcattcccg tgcgccggcg aggtgatagc aggggtagcc tgctttcgcc ccggcccatt
      481 tcctacttga aaggctcctc ggggggtccg ctgttgtgcc ccgcgggaca cgccgtgggc
      541 ctattcaggg ccgcggtgtg cacccgtgga gtggctaaag cggtggactt tatccctgtg
      601 gagaacctag agacaaccat gagatccccg gtgttcacgg acaactcctc tccaccagca
      661 gtcaccctga cgcacccaat caccaaaatc gataccaaat acatcatgac atgcatgtcg
      721 gccgacctgg aggtcgtcac gagcacctgg gtgctcgttg gcggcgtcct ggctgctctg
      781 gccgcgtatt gcctgtcaac aggctgcgtg gtcatagtgg gcaggatcgt cttgtccggg
      841 aagccggcag gcagtagcgg aagcagtatt atacctgaca gggaggttct ctaccaggag
      901 ttcgatgaga tg
//
