LOCUS       sequence-123868-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SMASh tag fused to YFP at N terminus in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, 
            Lin MZ
  TITLE     Tunable and reversible drug control of protein production via a 
            self-excising degron.
  JOURNAL   Nat Chem Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869.
  PUBMED    26214256
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869."
COMMENT     Sequence Label: pAG426GPD-ccdB-yeastSMASh-YFP
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     207..228
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
       61 gcactgcagg ccgtagccca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      121 ggtggtgcag atcagcttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      181 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      241 cccggtgaac agctcctcgc ccttgctcac cattaactgc tgagagcact cttccatctc
      301 atcgaactcc tggtagagaa cctccctgtc aggtataata ctgcttccgc tactgcctgc
      361 cggcttcccg gacaagacga tcctgcccac tatgaccacg cagcctgttg acaggcaata
      421 cgcggccaga gcagccagga cgccgccaac gagcacccag gtgctcgtga cgacctccag
      481 gtcggccgac atgcatgtca tgatgtattt ggtatcgatt ttggtgattg ggtgcgtcag
      541 ggtgactgct ggtggagagg agttgtccgt gaacaccggg gatctcatgg ttgtctctag
      601 gttctccaca gggataaagt ccaccgcttt agccactcca cgggtgcaca ccgcggccct
      661 gaataggccc acggcgtgtc ccgcggggca caacagcgga ccccccgagg agcctttcaa
      721 gtaggaaatg ggccggggcg aaagcaggct acccctgcta tcacctcgcc ggcgcacggg
      781 aatgacatcg gcgtgcctcg tgaccaggta aaggtccgag gagccgcagg tacagggtgt
      841 caatgagcgg gaacc
//
