LOCUS       sequence-123872-         547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas 
            fluorescens, codon optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Lucas JE, Siegel JB
  TITLE     Quantitative functional characterization of conserved molecular 
            interactions in the active site of mannitol 2-dehydrogenase.
  JOURNAL   Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015
            Apr 2.
  PUBMED    25752240
REFERENCE   2  (bases 1 to 547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2669"; 
            journalName: "Protein Sci"; date: "2015-06"; volume: "24"; issue: 
            "6"; pages: "936-45"
COMMENT     Sequence Label: pfMDH_K381A
FEATURES             Location/Qualifiers
     source          1..547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgaggc taccaacagg ttttttcagc
       61 agatgtttca gggtggtggt aacaccatta tcacgcaggc taccataaca acgttcaaat
      121 gcggcaacaa attccggact attcggaatt gcggtgccaa aaatttcttc aactgccagc
      181 agacgctggc taatcagtgc atcatctgaa accagaccct gacaaaattc tgcacgcgga
      241 tcaggaatgg tatagctcac accattttca tcaacgcctt tcagatacag tgcccatgct
      301 gcaacaacca gtgctgcacg ttcggtttca cgaccatctg caatcagacg attaattgtc
      361 ggaacggtaa atttaggaaa cgcgctgcta ccatcactac aaacacgttc cagctgatct
      421 gcaattgcct gattgctaaa acgatcaacc agggtctgtt tataatcggt cagatcaata
      481 cccggaaccg gagccagatt cggggtcaca tccagatcca tatatgcacg catataggca
      541 acaaaca
//
