LOCUS       sequence-123874-        7068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid encodes EGFP fused to the N-terminus of isoform 1 of 
            Cep123 (CCDC123/Cep89).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Sillibourne JE, Hurbain I, Grand-Perret T, Goud B, Tran P, Bornens M
  TITLE     Primary ciliogenesis requires the distal appendage component Cep123.
  JOURNAL   Biol Open. 2013 Apr 9;2(6):535-45. doi: 10.1242/bio.20134457. Print 
            2013 Jun 15.
  PUBMED    23789104
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/bio.20134457"; journalName: "Biol Open"; date: "2013-04-9- 
            9"; volume: "2"; issue: "6"; pages: "535-45"
COMMENT     Sequence Label: pEGFP-C1-Cep123 (CCDC123/Cep89)
FEATURES             Location/Qualifiers
     source          1..7068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3244..3365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3281..3300)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3335..3354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3372..3827)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3509..3530)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3721..3740
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3854..3958
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3960..4317
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3960..4151
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3983..4003)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4168..4303
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4352..5146
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4352..5146
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4406..4425)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5016..5035
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5334..5353)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5378..5425
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5754..6342
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6243..6262
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6517..6820
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6821..7024
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6975..6995
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      join(7063..7068,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc
      721 ggactcagat ctcgagctca agcttcgaat tctgcagtcg acatgctcct gggatttcgg
      781 agaggccgca ggagtcattt caaacacatc atccatggcc ttttacctgc agccagcgtt
      841 gctccgaagg cagctgtgcc acgcacacct cctccccgca gccccaaccc atctccagag
      901 agaccaagat ctgctctggc agcagccatt ctggcgacaa cattgactgg gcggacggtt
      961 gctattcctc agcctcgcca gaggtcccgg tctgagagtg atgtgagcag tgttgaacag
     1021 gacagcttca tcgagcccta tgccaccacc tcacagctga ggcctcgacc aaattggcag
     1081 agtgagatgg gaagaagatc ttctttgcca tcctttgaaa cactggacta tggggacgaa
     1141 gaggacattg aaactcagct gtcatccagc ggcaaggaat tgggggatgt cagtgcccgg
     1201 gaggacagag gaggccacag tgatgacctg tacgctgtgc cacacagaaa tcaggtgcca
     1261 ttgttacatg aggtgaacag tgaagacgat gaaaatattt ctcatcaaga tgggtttcca
     1321 ggctcccctc ctgcaccaca gcggacacaa caaaaagatg gtaaacaccc tgttctgaat
     1381 ttaaaggatg aaaaacctcc attatgtgag aaacctccac cctccccaga tataactggt
     1441 agagcacgtc aaagatatac agaaataacc agagaaaagt ttgaggcatt aaaagaagaa
     1501 aatatggacc taaacaatat gaatcaaagc cttacccttg aactaaacac aatgaaacaa
     1561 gcaatgaaag aactacagtt aaaacttaag ggaatggaaa aagagaagag aaagctcaaa
     1621 gaggctgaga aggcgtcgtc acaggaagtt gctgcacctg aattacttta tctgcgaaaa
     1681 caagctcaag aactggtgga tgaaaatgat ggattgaaaa tgactgtcca tcgtttgaat
     1741 gtagaactca gtcgatatca gacaaaattc aggcatttgt ccaaggaaga gagcttaaat
     1801 attgaaggcc tcccatccaa gggccctata ccaccctggt tgttggatat aaagtacctg
     1861 tcaccattgt tgctggctta tgaagatatg atgaaagaga aggacgagct caatgccacc
     1921 ctcaaggagg aaatgagaat gtttaggatg cgagtccaag aagtggtgaa agaaaatgaa
     1981 gaattgcacc aagagttaaa taagagtagt gctgttacca gtgaggaatg gcgtcagctt
     2041 cagactcaag caaaactggt tttagaggaa aacaagttgt tgctggagca gttggagatt
     2101 cagcaaagga aagccaagga cagccaccag gagcgcctcc aagaagtttc taagctgact
     2161 aaacaactaa tgctcctgga ggcaaaaacc cacggccagg aaaaggagct ggcggagaac
     2221 agggaacagc tggagatttt acgtgccaaa tgccaagaac tcaaaacaca ctcggatggc
     2281 aaaatcgcag tggaagttca taaatcaatt gtgaatgaat taaaaagcca attacagaag
     2341 gaagaagaga aagaaagggc tgagatggag gagttgatgg agaagctgac agtcctgcaa
     2401 gcgcagaaga agagcctgct gttagagaag aacagtttga cagagcaaaa caaagcactg
     2461 gaagccgaac ttgaacgagc acagaaaatc aataggaaat ctcaaaagaa aattgaggtc
     2521 ctcaaaaagc aggtggaaaa agccatgggg aacgaaatgt ctgctcatca gtacctggca
     2581 aaccttgttg gcctggcaga aaatataacc caggaacgtg acagtcttat gtgtttggca
     2641 aaatgtttag aaagtgagaa ggatggagtg cttaataaag tcataaaaag caacattcgc
     2701 ctgggaaagt tagaggaaaa agtcaagggc tacaagaagc aggcagcact gaagctgggg
     2761 gacatcagtc accgtctgct ggagcagcag gaggacttcg ccggcaagac agcccagtac
     2821 cggcaggaga tgcggcacct gcaccaggtg ctgaaggaca agcaggaggt gctggaccag
     2881 gcgctgcagc agaacagaga aatggaaggt gaacttgaag ttatttggga atctaccttc
     2941 agggaaaacc gaagaatccg agaacttctc caggacacac tcacgaggac aggcgtgcag
     3001 gacaacccca gagctctggt tgcccccagc ctcaatggcg tctctcaggc agacctgctg
     3061 gacggctgcg atgtctgctc ctatgacctg aagtctcatg cccccacctg ctagggatcc
     3121 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     3181 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     3241 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3301 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3361 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     3421 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3481 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3541 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3601 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3661 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3721 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3781 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     3841 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3901 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     3961 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4021 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4081 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4141 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4201 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4261 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     4321 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     4381 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4441 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4501 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     4561 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4621 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4681 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4741 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4801 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4861 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4921 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4981 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5041 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     5101 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     5161 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     5221 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     5281 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     5341 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     5401 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     5461 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     5521 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     5581 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     5641 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5701 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5761 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5821 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5881 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5941 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6001 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6061 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6121 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6181 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6241 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6301 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6361 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6421 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
     6481 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     6541 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     6601 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     6661 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     6721 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     6781 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     6841 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     6901 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     6961 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     7021 agctggttta gtgaaccgtc agatccgcta gcgctaccgg tcgccacc
//
