LOCUS       sequence-123887-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector; single gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ141A
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..90
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        127..430
                     /label=AtU6-1 promoter
                     /note="promoter for the Arabidopsis thaliana U6-1 snRNA 
                     gene (Waibel and Filipowicz, 1990)"
     misc_RNA        464..539
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtat acaaaagttg ccccatggcg ttccctctag ataacgcagg
      121 atcctaagaa atctcaaaat tccggcagaa caattttgaa tctcgatccg tagaaaccag
      181 acggtcattg ttttagttcc accacgatta tatttgaaat ttacgtgagt gtgagtgaga
      241 cttgcataag aaaataaaat ctttagttgg gaaaaaattc aataatataa atgggcttga
      301 gaaggaagcg agggataggc ctttttctaa aataggccca tttaagctat taacaatctt
      361 caaaagtacc acagcgctta ggtaaagaaa gcagctgagt ttatatatgg ttagacacga
      421 agtagtgatt ggagacgaga tctagtctga gtcgaccgtc tctgttttag agctagaaat
      481 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
      541 ttttttggca aaaattttca gattttttct tcatctgtag atttctgggt ttttttttcc
      601 gtttcgtgaa tcataagtga agttttggat gcaaatctgc gcgaaaaaag ttggacctgc
      661 aatgagctta tttagatagc taagacaaag tgattggtcc gttgtttcag ttctgattgt
      721 cagagagttt gtttcgagtc ggcgacacca atgcgttttg ttaaccagat ttcgggtaag
      781 aaatgtatcg agagtttgtt tccagacggc tacatcattt tcttatgaag ggtgaaatta
      841 gatagaccaa agattgaaac aca
//
