LOCUS       sequence-123888-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector; single gRNA under AtU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ141B
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      183..771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     672..691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     925..942
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
       61 ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt
      121 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
      181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
      241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
      301 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
      361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      961 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     1021 tttcccgact ggaaagcggg cagtgagc
//
