LOCUS       sequence-123889-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector; single gRNA under AtU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ141B
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..88
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_RNA        484..559
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(762..786)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtatac aaaagttgcc ccatggcgtt ccctctagat aacgcaggat
      121 cctatttact ttaaattttt cttatggctc agcctgtgat ggataactga atcaaacaaa
      181 tggcgtctgg gtttaagaac atctgttttg gctatgttgg acgaaacaag tgaactttta
      241 ggatcaactt ccgtttatat acggagctta tatcgagcaa taagataagt gggcttttta
      301 tgtaatttaa tgggctatcg tccatatatt cactaatacc catgcccagt acccatgtat
      361 gcgtttcata taagctccta atttctccca catcgctcaa atctaaacaa atcttgttgt
      421 atatataaca ctgagggagc accattggtc agagacgaga tctagtctga gtcgaccgtc
      481 tctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      541 agtggcaccg agtcggtgct tttttttccc tttccttttt tctttttttt gccataaact
      601 taaatttgta tatcgatcat tgtagatatt gaaaacctag aacaaaccaa catccatgtg
      661 aatgtctttc atgactgatt tagagataat tcttgaattt tggaactaga atctataatg
      721 agcctaaatt aaaacattgt gaactagtaa gggcgaattc gacccagctt tcttgtacaa
      781 agttggcatt ataaaaaata attgctcatc aatttgttgc aacgaacagg tca
//
