LOCUS       sequence-123903-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mTFP1 using KanMX6 as a selection marker in 
            S. cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: pFA6-mTFP1-KanMX6
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..723
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 ttaattaaca gtatggtgag caagggcgag gagaccacaa tgggcgtaat caagcccgac
       61 atgaagatca agctgaagat ggagggcaac gtgaatggcc acgccttcgt gatcgagggc
      121 gagggcgagg gcaagcccta cgacggcacc aacaccatca acctggaggt gaaggaggga
      181 gcccccctgc ccttctccta cgacattctg accaccgcgt tcgcctacgg caacagggcc
      241 ttcaccaagt accccgacga catccccaac tacttcaagc agtccttccc cgagggctac
      301 tcttgggagc gcaccatgac cttcgaggac aagggcatcg tgaaggtgaa gtccgacatc
      361 tccatggagg aggactcctt catctacgag atacacctca agggcgagaa cttccccccc
      421 aacggccccg tgatgcagaa gaagaccacc ggctgggacg cctccaccga gaggatgtac
      481 gtgcgcgacg gcgtgctgaa gggcgacgtc aagcacaagc tgctgctgga gggcggcggc
      541 caccaccgcg ttgacttcaa gaccatctac agggccaaga aggcggtgaa gctgcccgac
      601 tatcactttg tggaccaccg catcgagatc ctgaaccacg acaaggacta caacaaggtg
      661 accgtttacg agagcgccgt ggcccgcaac tccaccgacg gcatggacga gctgtacaag
      721 taaggcgcgc c
//
