LOCUS       sequence-123912-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  regulated expression of genes in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Stark MJ
  TITLE     Multicopy expression vectors carrying the lac repressor gene for 
            regulated high-level expression of genes in Escherichia coli.
  JOURNAL   Gene. 1987;51(2-3):255-67.
  PUBMED    3110013
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1987"; volume: "51(2-3)"; pages: "255-67"
COMMENT     Sequence Label: pttq18
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(44..61)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(44..60)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(53..75)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(291..308)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      461..547
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc gtcgttttac
       61 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      121 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      181 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
      241 tttcacaccg cataaattcc ctgttttggc ggatgagaga agattttcag cctgatacag
      301 attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg
      361 gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt
      421 gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
      481 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      541 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
      601 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgac
//
