LOCUS       sequence-123913-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP and FLAG-tagged AKAP-Lbc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Burmeister BT, Wang L, Gold MG, Skidgel RA, O'Bryan JP, Carnegie GK
  TITLE     Protein Kinase A (PKA) Phosphorylation of Shp2 Protein Inhibits Its 
            Phosphatase Activity and Modulates Ligand Specificity.
  JOURNAL   J Biol Chem. 2015 May 8;290(19):12058-67. doi: 
            10.1074/jbc.M115.642983. Epub 2015 Mar 23.
  PUBMED    25802336
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.642983"; journalName: "J Biol Chem"; date: 
            "2015-05-8- 8"; volume: "290"; issue: "19"; pages: "12058-67"
COMMENT     Sequence Label: pEGFP-FLAG-AKAP-Lbc
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             81..128
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 ccgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgccaccatg gactacaagg acgacgatga caaggactac aaggacgacg
      121 atgacaaggg taccgccacc atgaaactta atccacagca agctccctta tatggtgatt
      181 gtgttgttac agtgctgctt gctgaagagg acaaagctga agatgatgta gtgttttact
      241 tggtattttt gggttccacc ctccgtcact gtacaagtac tcggaaggtc agttctgata
      301 cattggagac cattgctcct ggtcatgatt gttgtgaaac agtgaaggtg cagctctgtg
      361 cttccaaaga gggccttccc gtgtttgtgg tggctgaaga agactttcat ttcgtccagg
      421 atgaagcgta tgatgcagct caattcctag caaccagtgc tggaaatcag caggctttga
      481 actttacccg ttttcttgac cagtcaggac ccccatctgg ggatgtgaat tcccttgata
      541 agaagttggt gctggcattc aggcacctga agctgcccac ggagtggaat gtattgggga
      601 cagatcagag tttgcatgat gctggcccgc gagagacatt gatgcatttt gctgtgcggc
      661 tgggactgct gaggttgacg tggttcctgt tgcagaagcc aggtggccgc ggagctctca
      721 gtatccacaa ccaggaagg
//
