LOCUS       sequence-123914-       11856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a fluorescent chitin reporter under Gal4 control in 
            Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11856)
  AUTHORS   Sobala LF, Wang Y, Adler PN
  TITLE     ChtVis-Tomato, a genetic reporter for in vivo visualization of 
            chitin deposition in Drosophila.
  JOURNAL   Development. 2015 Nov 15;142(22):3974-81. doi: 10.1242/dev.126987. 
            Epub 2015 Sep 22.
  PUBMED    26395478
REFERENCE   2  (bases 1 to 11856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.126987"; journalName: "Development"; date: "2015-11-15-
            15"; volume: "142"; issue: "22"; pages: "3974-81"
COMMENT     Sequence Label: pWalium-ChtVis-Tomato
FEATURES             Location/Qualifiers
     source          1..11856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1448..2062,2133..2264,2485..2800,2862..3516,
                     3591..3864,4259..4330))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4790..4808
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    4927..4996
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    5234..5664
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5682..5715
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5728..5822
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5838..5871
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5884..5978
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        6002..6240
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6187..6208
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             6359..7786
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             7793..7948
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     intron          8592..8657
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8787..8807
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9079..9200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9116..9135)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9170..9189
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9222..9652
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     promoter        complement(9671..9689)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9710..9726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9710..9726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9723..9745)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9734..9750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9758..9764,9765..9782,9783..9788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9803..9824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9941..9958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10112..10700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10192..10211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10871..11662,11663..11731))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11494..11513
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11732..11836)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacaag
      661 cttcacgacc taggcgcgcc ctagttccag tgaaatccaa gcattttcta aattaaatgt
      721 attcttatta ttatagttgt tatttttgat atatataaac aacactatta tgcccaccat
      781 ttttttgaga tgcatctaca caaggaacaa acactggatg tcactttcag ttcaaattgt
      841 aacgctaatc actccgaaca ggtcacaaaa aattacctta aaaagtcata atattaaatt
      901 agaataaata tagctgtgag ggaaatatat acaaatatat tggagcaaat aaattgtaca
      961 tacaaatatt tattactaat ttctattgag acgaaatgaa ccactcggaa ccatttgagc
     1021 gaaccgaatc gcgcggaact aacgacagtc gctccaaggt cgtcgaacaa aaggtgaatg
     1081 tgttgcggag agcgggtggg agacagcgaa agagcaacta cgaaacgtgg tgtggtggag
     1141 gtgaattatg aagagggcgc gcgatttgaa aagtatgtat ataaaaaata tatcccggtg
     1201 ttttatgtag cgataaacga gtttttgatg taaggtatgc aggtgtgtaa gtcttttggt
     1261 tagaagacaa atccaaagtc tacttgtggg gatgttcgaa ggggaaatac ttgtattcta
     1321 taggtcatat cttgttttta ttggcacaaa tataattaca ttagcttttt gagggggcaa
     1381 taaacagtaa acacgatggt aataatggta aaaaaaaaaa caagcagtta tttcggatat
     1441 atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc cagatatgcg agcacccgga
     1501 agctcacgat gagaatggcc agacccacgt agtccagcgg cagatcggcg gcggagaagt
     1561 taagcgtctc caggatgacc ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc
     1621 taatttcgcc cggctccacg tccgcccatt ggttaatcag cagaccctcg ttggcgtaac
     1681 ggaaccatga gaggtacgac aaccatttga ggtatactgg caccgagccc gagttcaaga
     1741 agaagccgcc aaagagcagg aatggtatga taaccggcgg acccacagac agcgccatcg
     1801 aggtcgagga gctggcgcag gatattagat atccgaagga cgttgacaca ttggccacca
     1861 gagtgaccag cgccaggcag ttgaagaagt gcagcactcc ggcccgcagt ccgatcatcg
     1921 gataggcaat cgccgtgaag accagtggca ctgtgagaaa aagcggtaat tcggcaatcg
     1981 ttttgcccag aaagtatgtg tcacagcgat aaagtcgact tcgggcctcc ctcataaaaa
     2041 ctggcagctc tgaggtgaac acctaaatcg aatcgattca ttagaaagtt agtaaattat
     2101 taatatgcaa atgtattcta aacaagactt acatttatcg tggcaaagac gttttgaaag
     2161 gtcatgttgg tcaggaagag gaagatggct ccgttgatat tcatcacgcc cacttgcgtg
     2221 agttgttggc ccaaaaagat gaggccaatc aagatggcaa ccatctgcaa attaaaatgt
     2281 tactcgcatc tcattaatat tcatatcttc aacatgttcg cgagttaaat gaaatttatt
     2341 tattttctgc aaaactataa actatacatc tcattgaaaa aaactaagaa gggtgtggaa
     2401 tcaggcaatt ctaactaaaa tctagcgaat ttgtttccaa gaattgtaag cgttatatca
     2461 tttgtttcca ctggaaccac tcaccgttgt ctgaataagt cgcactttta cgaggagtgg
     2521 ttccttgagc accgacagcc aggatcgcca caggaccgcc cggaactgca tgaaccaggt
     2581 ggccttgtag gtgtacccat tctccggctg ctccagtggc ttctccaaat ttttggtggc
     2641 caacaactgc tccatatccc gggctacttt gctaatagca aaattgtcgc atatcttggc
     2701 gatccgatca cgggactcga tctcccgtcc gggcacaacg gccaacacct gtacgtaaaa
     2761 gtccgccgga ttgtagttgg taggacactg ggcacccacg ctggatagga gttgagatgt
     2821 aatgtaatgc tagataccct taataaacac atcgaactca ctaggaaaag aagtcgacgg
     2881 cttcgctggg agtgcccaag aaagctaccc tgccctcggc catcagaagg atcttgtcaa
     2941 agagctcaaa cagctcggaa gacggctgat gaatggtcag gatgacggtc ttgcccttct
     3001 gcgacagctt cttcagcacc tggacgacgc tgtgggcggt aaatgagtcc agtccggagg
     3061 tgggctcatc gcagatcaga agcggcggat cggttagtgc ctcggaggcg aatgccagac
     3121 gcttcctttc tccgccggac agacctttca ccctgccggg cacaccgatg atcgtgtgct
     3181 gacatttgct gagcgaaagc tcctggatca cctgatccac gcgggccact cgctgccgat
     3241 aggtcagatg tcgtggcatc cgcaccatgg cctggaaaat caggtgttcc ctggccgtta
     3301 gggagccgat aaagaggtca tcctgctgga cataggcgca cctggcctgc atctccttgg
     3361 cgtccacagg ttggccattg agcagtcgca tcccggatgg cgatacttgg atgccctgcg
     3421 gcgatcgaaa ggcaagggca ttcagcaggg tcgtctttcc ggcaccggaa ctgcccatca
     3481 cggccaaaag ttcgcccgga taggccacgc cgcaaactga gtttcaaatt ggtaattgga
     3541 ccctttatta agatttcaca cagatcagcc gactgcgaat agaaactcac cgttcttgag
     3601 caaatgtttc ctgggcgccg gtatgtgtcg ctcgttgcag aatagtccgc gtgtccggtt
     3661 gaccagctgc cgccatccgg agcccggctg attgaccgcc ccaaagatgt ccatattgtg
     3721 ccaggcatag gtgaggttct cggctagttg gccgctccct gaaccggagt cctccggcgg
     3781 actgggtggc aggagcgtgc cgtagttttt ggcctgcccg aagccctggt taatgcagct
     3841 ctgcgaagcc gctccgctgt caccctgcaa tgatagggga tctcaaatat caactacaag
     3901 cgttatgctc atctaacccc gaacaaaacg aagtatccta cgaagtaggt ttatactttt
     3961 atttattttt tgtgcatcta ggatcagctt aaaatatctg gttgttatat tttttgtaaa
     4021 aaagaatgta gtcgaaaatg aatgccttta gatgtcttga tcatgatatg atcttaaaaa
     4081 ttgtcttata tagcgagcac agctaccaga ataatctgtt tcgtgtcact atttgtttgt
     4141 gcgattgcgg tttgggattt ttgtgggtcg cagttctcac gccgcagaca atttgatgtt
     4201 gcaatcgcag ttcctataga tcaagtgaac ttaagatgta tgcacatgta ctactcacat
     4261 tgttcagatg ctcggcagat gggtgtttgc tgcctccgcg aattaatagc tcctgatcct
     4321 cttggcccat tgccgggatt tttcacactt tcccctgctt acccacccaa aaccaatcac
     4381 caccccaatc actcaaaaaa caaacaaaaa taagaagcga gaggagtttt ggcacagcac
     4441 tttgtgttta attgatggcg taaaccgctt ggagcttcgt cacgaaaccg ctgacaaagt
     4501 gcaactgaag gcggacattg acgctaggta acgctacaaa cggtggcgaa agagatagcg
     4561 gacgcagcgg cgaaagagac ggcgatattt ctgtggacag agaaggaggc aaacagcgct
     4621 gactttgagt ggaatgtcat tttgagtgag aggtaatcga aagaacctgg tacttcaaat
     4681 acccttggat cgaagtaaat ttaaaactga tcagataagt tcaatgatat ccagtgcagt
     4741 aaaaaataaa aaaaaaaaat gttttttttt atctactttc cgcaaaaatg ggttttatta
     4801 acttacatac atggcgcgcc agatcgcaag aagcttgata tcatcgatct cgaggctgca
     4861 tccaacgcgt tgggagctct ccggatcaat tcggcttcag gtaccgtcga cgatgtaggt
     4921 cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta
     4981 ctccacctca cccatctggt ccatcatgat gaacgggtcg aggtggcggt agttgatccc
     5041 ggcgaacgcg cggcgcaccg ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac
     5101 ggtgagcacg ggacgtgcga cggcgtcggc tggtgcggat acgcggggca gcgtcagcgg
     5161 gttctcgacg gtcacggcgg gcatgtcgac aagccgaatt gatccactag aaggcctaat
     5221 tcggtacact agttggccac gtaataagtg tgcgttgaat ttattcgcaa aaacattgca
     5281 tattttcggc aaagtaaaat tttgttgcat accttatcaa aaaataagtg ctgcatactt
     5341 tttagagaaa ccaaataatt ttttattgca tacccgtttt taataaaata cattgcatac
     5401 cctcttttaa taaaaaatat tgcatacttt gacgaaacaa attttcgttg catacccaat
     5461 aaaagattat tatattgcat acccgttttt aataaaatac attgcatacc ctcttttaat
     5521 aaagaatatt gcatacgttg acgaaacaaa ttttcgttgc atacccaata aaagattatt
     5581 atattgcata ccttttcttg ccataccatt tagccgatca attgtgctcg gcaacagtat
     5641 atttgtggtg tgccaaccaa caacactagt agtaccagct tataacttcg tataatgtat
     5701 gctatacgaa gttatctgca ggcaggtcgg agtactgtcc tccgagcgga gtactgtcct
     5761 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     5821 agcggagact cccatggata acttcgtata atgtatgcta tacgaagtta tggatccgca
     5881 ggtcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
     5941 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagactcgtc gacagcgagc
     6001 gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa
     6061 gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac
     6121 aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa
     6181 tcaactgcaa ctactgaaat ctgccaagaa gtaattattg aatacaagaa gagaactctg
     6241 aatagggaat tgggaattca aaatgggaca cctgacgcga cgcagcagcc taatggccat
     6301 agtcctgtgc ctggtactca acacacagca cctgtcggtg catggcgatg cctcgcatat
     6361 ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     6421 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     6481 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     6541 cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     6601 cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     6661 ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     6721 caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     6781 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     6841 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     6901 ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga
     6961 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     7021 ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct ccggcaccgc
     7081 ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca aggtgcgcat
     7141 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     7201 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     7261 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
     7321 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     7381 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     7441 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     7501 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     7561 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     7621 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
     7681 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     7741 gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagatct atacgacaaa
     7801 tcctggtgta tccgcttggc aggtcaacac agcttatact gcgggacaat tggtcacata
     7861 taacggcaag acgtataaat gtttgcagcc ccacacctcc ttggcaggat gggaaccatc
     7921 caacgttcct gccttgtggc agcttcaagg tgcctcgaga aggcgcagag acaccatgga
     7981 cattgtggtg aagccacaga ggatctacaa gaggaacgcg caggagatga ccgatgtgaa
     8041 caccagccga attatccagg tggtggcgcc cggagacgtt aactttgcgc tgaacagcaa
     8101 tgccagcaac gagacggtgg tcatccagtc ggccaggtcc gcggatgcgg agaccatctg
     8161 catgtcggtg cccagctttg tgggcggatt ggtgatgctg ctgctcgtcc tggccgtcgc
     8221 ctctctagtc gccgccttcc tcttcgtccg cgtgcgtcac ttcgatcgca agggagcggg
     8281 catggcctat gtgaactaat ctagagcaaa ctagttctga tctgctagac aattgttggc
     8341 atcaggtagg catcacacac gattaacaac ccctaaaaat acactttgaa aatattgaaa
     8401 atatgttttt gtatacattt ttgatatttt caaataatac gcagttataa aactcattag
     8461 ctaacccatt ttttctttgc ttatgcttac agattgcaaa gaactagagc cgcgggatct
     8521 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
     8581 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
     8641 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
     8701 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
     8761 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
     8821 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
     8881 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
     8941 aaaaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     9001 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     9061 aatggaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9121 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9181 aaactcatca atgtatctta tcatgtctgg ttccagagct ctggccacgt aataagtgtg
     9241 cgttgaattt attcgcaaaa acattgcata ttttcggcaa agtaaaattt tgttgcatac
     9301 cttatcaaaa aataagtgct gcatactttt tagagaaacc aaataatttt ttattgcata
     9361 cccgttttta ataaaataca ttgcataccc tcttttaata aaaaatattg catactttga
     9421 cgaaacaaat tttcgttgca tacccaataa aagattatta tattgcatac ccgtttttaa
     9481 taaaatacat tgcataccct cttttaataa agaatattgc atacgttgac gaaacaaatt
     9541 ttcgttgcat acccaataaa agattattat attgcatacc ttttcttgcc ataccattta
     9601 gccgatcaat tgtgctcggc aacagtatat ttgtggtgtg ccaaccaaca acgagctcca
     9661 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
     9721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9841 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9901 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    10561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10741 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10801 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10861 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10921 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10981 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    11041 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    11101 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    11161 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    11221 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11281 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11341 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    11401 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    11461 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    11521 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    11581 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    11641 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11701 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11761 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11821 aaataggggt tccgcgcaca tttccccgaa aagtgc
//
