LOCUS       sequence-123920-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-tagged Shp2 T73D/S189D SH2 domains in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Burmeister BT, Wang L, Gold MG, Skidgel RA, O'Bryan JP, Carnegie GK
  TITLE     Protein Kinase A (PKA) Phosphorylation of Shp2 Protein Inhibits Its 
            Phosphatase Activity and Modulates Ligand Specificity.
  JOURNAL   J Biol Chem. 2015 May 8;290(19):12058-67. doi: 
            10.1074/jbc.M115.642983. Epub 2015 Mar 23.
  PUBMED    25802336
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.642983"; journalName: "J Biol Chem"; date: 
            "2015-05-8- 8"; volume: "290"; issue: "19"; pages: "12058-67"
COMMENT     Sequence Label: pGEX-4T-1-Shp2 T7DA/S189D SH2s
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(710..727)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(754..776)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gcagatcgtc agtcagtcac gatgcggccg ctcgagtcga cccggtgttg aggggctgct
       61 tgagctgcag gactgtgccc agcgtctcca ccatggggtt cttcttgtaa tgctccacca
      121 ggtctgtcag atcgtcaaag cgctctcccc caccaacgtc gtatttcagc tcctgacagc
      181 ggatcatgac gtgggtcact ttggacttgc cgtcgttgct ctcccctttg tcgtcaccag
      241 tgcgcacgga gagaacgaag tctccggggt ggctctggct ctctcgaacg aggaagctgc
      301 catgcttgcc cttctccgtc agcagcttct ctgcttcttt tccagacaag tgaccatgga
      361 accacctttc agaggtaggg tctgcacagt tcagcgggta cttgagctcg ataacatctc
      421 cattcttctc tttcagctgc ccatggtgtt ccatgtaata ctgaaccagt tcagccaaat
      481 cggcaaattt ctccccacca tagaggtcat agtagtcccc agtgttctga atcttgatgt
      541 gggtaacagc tccatttctt ctaacagaca gagtgaagtc tccagggtta ctcttactgg
      601 gccttgctaa aaaactgcca tcgactcctc tggtcagcag gagattctct gcctccacac
      661 cagtgatgtt ggggtgaaac catctccgcg atgtcatccg gaattccggg gatccacgcg
      721 gaaccagatc cgattttgga ggatggtcgc caccaccaaa cgtggcttgc cagccctgca
      781 aaggccatgc tatatacttg ctggatttca agtacttatc aatttgtggg atagcttcaa
      841 tacgtt
//
