LOCUS       sequence-123922-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the UCR1 region of PDE4D3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Wang L, Burmeister BT, Johnson KR, Baillie GS, Karginov AV, Skidgel 
            RA, O'Bryan JP, Carnegie GK
  TITLE     UCR1C is a novel activator of phosphodiesterase 4 (PDE4) long 
            isoforms and attenuates cardiomyocyte hypertrophy.
  JOURNAL   Cell Signal. 2015 May;27(5):908-22. doi: 
            10.1016/j.cellsig.2015.02.003. Epub 2015 Feb 12.
  PUBMED    25683917
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cellsig.2015.02.003"; journalName: "Cell Signal"; date: 
            "2015-05"; volume: "27"; issue: "5"; pages: "908-22"
COMMENT     Sequence Label: FLAG-Cerulean-UCR1
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(433..684)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(475..496)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     824..843
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcactgggga ggggtcacag ggatgccacc cgggatcctc tagagtcgac tggtacctca
       61 tcaggcctcc tctgttatgg tggctttgtt gatggatggt tggttgcaca tgggtgatct
      121 tttgctaggt gctcgatctt gcaaattagt taatgcagca aagttgtttc gtacagttcg
      181 cagactggcc aagacctgag caaatggagt cacaatcaag tcatctccgt gtatatcact
      241 ggcaatggag gagttccggg acatagactt tggagagagg tcataatcgc tgtcggatcg
      301 atacaggaag gactcccgtc gttgactgtg gacaaaattt gcttggagaa ttagcccgga
      361 tcctgggctg gtcatgggat ccaagggact ccgtcccgca gatgtgccat tgtccacatc
      421 aaaggaattc gccttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      481 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgggt
      541 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      601 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttggc
      661 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtcgctga tggcgttgta
      721 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      781 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      841 gtccttg
//
