LOCUS       sequence-123926-        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ144
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(94..885)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgtaactcac
       61 gttaagggat tttggtcatg ggtggctcga gggttatttg ccgactacct tggtgatctc
      121 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
      181 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
      241 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
      301 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
      361 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
      421 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
      481 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
      541 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
      601 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
      661 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
      721 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
      781 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
      841 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactttgt
      901 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
      961 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
     1021 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     1081 tctggaccag ttgcgtgagc gcatacgcta cttgcatta
//
