LOCUS       sequence-123933-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate recipient and Gateway entry vector; assembly of 5 gRNAs 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ145
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(100..891)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgacgcgta
       61 actcacgtta agggattttg gtcatgggtg gctcgagggt tatttgccga ctaccttggt
      121 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
      181 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
      241 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
      301 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
      361 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
      421 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
      481 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
      541 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
      601 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
      661 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
      721 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
      781 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
      841 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca taatgtttaa
      901 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
      961 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
     1021 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccacc
//
