LOCUS       sequence-123935-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Durandau E, Aymoz D, Pelet S
  TITLE     Dynamic single cell measurements of kinase activity by synthetic 
            kinase activity relocation sensors.
  JOURNAL   BMC Biol. 2015 Aug 1;13:55. doi: 10.1186/s12915-015-0163-z.
  PUBMED    26231587
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2015-08-1"; volume: "13:55. doi"; pages: " 
            10.1186/s12915-015-0163-z"
COMMENT     Sequence Label: pED73 pRS304 pRPS2-SteDS-NLS-mCitrine
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accaaaattg ggacaacacc agtgaataat tcttcacctt tagaagcaga tctaaccaaa
       61 ccagaagccg tatctggtga tatttttagt ttttttagcg gggaacctag ttcttcaaat
      121 tccgaaccac gacccatttg ttggaattca accaaaccag aagccgtatc tggtgatatt
      181 tttagttttt ttagcgggga acctagttct tcaaattccg aaccacgacc catttgttgt
      241 ctagatgtct tttcctgcaa ttggccatta ttgcccacat ctgggtgcag gttaagattg
      301 agccctttca agttccttct ctgtaaagtc tttcgttgaa acataagctt tacttaatta
      361 gttgagctta ttggttgttt gttatagtcg gaatggaaca cggaaagttg atgtaggtaa
      421 ctttcaatcg acaatatata aagaacacgg tttctttggg attgaaaagg tatcaagcag
      481 taaggaagga aattggaaac aaaggaagag aaagtctgaa ttaaaaactt gaaaactctt
      541 acgttactac agaggggaat agatgaaaaa atagctaggc gtcctgggaa gctgcgtgca
      601 atactaaggc aggacagata cctacgttac ctcgttaatg atgacatgcg ttttccatca
      661 aatgtttgcg tccagttcca ttatcacaaa tcttaaaatt caaacaagta aataatccca
      721 tagagcgggt gtatgggtct agcttaaata cacccaacca tatttagaaa gcagtcttct
      781 aa
//
