LOCUS       sequence-123937-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ146
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(97..888)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
       61 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
      121 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
      181 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
      241 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
      301 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
      361 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
      421 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
      481 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
      541 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
      601 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
      661 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
      721 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
      781 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
      841 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
      901 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
      961 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     1021 acagtcataa caagcc
//
