LOCUS       sequence-123941-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 1st gRNA under OsU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ131D
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(352..371)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(353..371)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(376..392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(376..392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        481..858
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        891..966
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat
       61 ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata
      121 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
      181 ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc
      241 ttgcggagaa ctgtgaatgc gcaaaccaac ccttgatcgg ggaagaacag tatgtcgagc
      301 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat cccctatagt
      361 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
      421 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagag gtctcgctat
      481 aagggatctt taaacatacg aacagatcac ttaaagttct tctgaagcaa cttaaagtta
      541 tcaggcatgc atggatcttg gaggaatcag atgtgcagtc agggaccata gcacaggaca
      601 ggcgtcttct actggtgcta ccagcaaatg ctggaagccg ggaacactgg gtacgttgga
      661 aaccacgtga tgtggagtaa gataaactgt aggagaaaag catttcgtag tgggccatga
      721 agcctttcag gacatgtatt gcagtatggg ccggcccatt acgcaattgg acgacaacaa
      781 agactagtat tagtaccacc tcggctatcc acatagatca aagctggttt aaaagagttg
      841 tgcagatgat ccgtggcaga gacgagatct agtctgagtc gaccgtctct gttttagagc
      901 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      961 cggtgctttt tttgtccctt cgaagggcaa ttctgcagat atccatcaca ctggcggccg
     1021 ctcgaggtcg agggtatcga taagcttcat gggagaccct cgagccaccc atgaccaaaa
     1081 tccctta
//
