LOCUS       sequence-123942-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 1st gRNA under OsU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ131D
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(188..263)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(296..673)
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     primer_bind     762..778
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     762..778
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     783..802
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        783..801
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gacgcgtaac
       61 tcacgttaag ggattttggt catgggtggc tcgagggtct cccatgaagc ttatcgatac
      121 cctcgacctc gagcggccgc cagtgtgatg gatatctgca gaattgccct tcgaagggac
      181 aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa
      241 cttgctattt ctagctctaa aacagagacg gtcgactcag actagatctc gtctctgcca
      301 cggatcatct gcacaactct tttaaaccag ctttgatcta tgtggatagc cgaggtggta
      361 ctaatactag tctttgttgt cgtccaattg cgtaatgggc cggcccatac tgcaatacat
      421 gtcctgaaag gcttcatggc ccactacgaa atgcttttct cctacagttt atcttactcc
      481 acatcacgtg gtttccaacg tacccagtgt tcccggcttc cagcatttgc tggtagcacc
      541 agtagaagac gcctgtcctg tgctatggtc cctgactgca catctgattc ctccaagatc
      601 catgcatgcc tgataacttt aagttgcttc agaagaactt taagtgatct gttcgtatgt
      661 ttaaagatcc cttatagcga gacctctaga ggaggcatga tgatatattt ttatcttgtg
      721 caatgtaaca tcagagattt tgagacacgg gccagagctg ccaggaaaca gctatgacca
      781 tgtaatacga ctcactatag gggatatttt attttctccc aatcaggctt gatccccagt
      841 aagtcaaaaa atagctcgac atactgttct tccccgatca agggttggtt tgcgcattca
      901 cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt
      961 gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg
     1021 gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc
     1081 cgaggcggca taaatcgccg tgacgatc
//
