LOCUS       sequence-123944-         565 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under
            the inducible BAD promoter and a single copy of the endonuclease 
            cleavage site which is readily extendable to multiple copies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Chen Z, Zhao H
  TITLE     A highly sensitive selection method for directed evolution of homing
            endonucleases.
  JOURNAL   Nucleic Acids Res. 2005 Oct 6;33(18):e154.
  PUBMED    16214805
REFERENCE   2  (bases 1 to 565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005-10-6"; volume: "33(18)"; pages: "e154"
COMMENT     Sequence Label: p11-LacY-wtx1
FEATURES             Location/Qualifiers
     source          1..565
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..361
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     240..259
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(456..473)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcgattga aaacgatgca
       61 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
      121 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
      181 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
      241 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
      301 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgtttt ggggaatata
      361 atctagcatt acgctaggga taacagggta atatcacgct ctagacatac ggcatgcaag
      421 cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc
      481 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
      541 cccatgccga actcagaagt gaaac
//
