LOCUS       sequence-123946-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with hSpCas9D10A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ159
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             129..194
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             201..221
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg ctccgaattc gcccttcacc atgaccggtg
      121 ccaccatgga ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag
      181 acgatgacga taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag
      241 cagccgacaa gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg
      301 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
      361 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
      421 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
      481 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca
      541 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg
      601 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa
      661 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc
      721 acatgatcaa gttccggtgc cacttcctga tcgagggcga cctgaacccc gacaacagcg
      781 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc
      841 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca
      901 gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca
      961 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
     1021 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg
//
