LOCUS       sequence-123950-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with hSpCas9D10A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ159
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(12..135)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(180..432)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(454..501)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cagctggatg gcatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
       61 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt
      121 ttgtatacaa agttgcgaat tcgccctttc cggaagctgt cgagggggga tcaattcccg
      181 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
      241 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
      301 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
      361 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
      421 gtttgaacga tcggacgttt atcacaattc ttactttttc ttttttgcct ggccggcctt
      481 tttcgtggcc gccggccttt tgtcgcctcc cagctgagac aggtcgatcc gtgtctcgta
      541 caggccggtg atgctctggt ggatcagggt ggcgtccagc acctctttgg tgctggtgta
      601 cctcttccgg tcgatggtgg tgtcaaagta cttgaaggcg gcaggggctc ccagattggt
      661 cagggtaaac aggtggatga tattctcggc ctgctctctg atgggcttat cccggtgctt
      721 gttgtaggcg gacagcactt tgtccagatt agcgtcggcc aggatcactc tcttggagaa
      781 ctcgctgatc tgctcgatga tctcgtccag gtagtgcttg tgctgttcca caaacagctg
      841 tttctgctca ttatcctcgg gggagccctt cagcttctca tagtggctgg ccaggtacag
      901 gaagttcaca tatttggagg gcagggccag ttcgtttccc ttctgcagtt cgccggcaga
      961 ggccagcatt ctcttccggc cgttttccag ctcgaacagg gagtacttag gcagcttgat
     1021 gatcaggtcc tttttcactt ctt
//
