LOCUS       sequence-123951-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with Cas9p.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ167
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(13..136)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(181..433)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(481..528)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcagctggat ggcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
       61 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact
      121 tttgtataca aagttgcgaa ttcgcccttt ccggaagctg tcgagggggg atcaattccc
      181 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
      241 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
      301 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
      361 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
      421 tgtttgaacg atcggacgtc accggtgttt aaacgctagc tctagatcag gtggatccta
      481 cttctttttc ttagcctgtc cggccttttt ggtggcagca ggacgcttat caccaccaag
      541 ctgggaaagg tcgatacgag tctcgtaaag accggtgatg gactggtgga tgagagtagc
      601 gtcgagaacc tccttggtgg acgtgtaacg cttcctgtcg atggtggtgt cgaagtactt
      661 gaaagcagca ggggcgccga ggttcgtgag cgtgaagagg tggatgatgt tctcggcctg
      721 ctcgcggatg ggcttgtcgc ggtgcttgtt gtaggcggag aggaccttgt cgaggttagc
      781 gtcagcgagg atgacgcgct tggagaactc ggagatctgc tcgatgatct cgtcgaggta
      841 gtgcttgtgc tgctccacga agagctgctt ctgctcgtta tcctcagggg aacccttgag
      901 cttctcgtag tgggaggcga ggtagaggaa gttcacgtac ttggaaggaa gagcaagctc
      961 gttacccttc tggagctcac cagcggaagc cagcatcctc ttacgaccgt tctcgagctc
     1021 gaaaagagag tacttgggga gcttgatgat gaggtccttc ttgacctcct tgtagccctt
     1081 gg
//
