LOCUS       sequence-123952-        7376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with Cas9p.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ167
FEATURES             Location/Qualifiers
     source          1..7376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2585..2605
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2630..6730
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2630..6730
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             3816..3824
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6731..6778
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      6826..7078
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7123..7246
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7263..7282)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7264..7282)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7287..7303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7287..7303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
     2581 ggctcctaag aagaagcgga aggttggtat tcacggggtg cctgcggctg acaagaagta
     2641 ctccatcggc ctcgacatcg gcaccaacag cgtcggctgg gcggtgatca ccgacgagta
     2701 caaggtcccg tccaagaagt tcaaggtcct gggcaacacc gaccgccact ccatcaagaa
     2761 gaacctcatc ggcgccctcc tcttcgactc cggcgagacg gcggaggcga cccgcctcaa
     2821 gcgcaccgcc cgccgccgct acacccgccg caagaaccgc atctgctacc tccaggagat
     2881 cttctccaac gagatggcga aggtcgacga ctccttcttc caccgcctcg aggagtcctt
     2941 cctcgtggag gaggacaaga agcacgagcg ccaccccatc ttcggcaaca tcgtcgacga
     3001 ggtcgcctac cacgagaagt accccactat ctaccacctt cgtaagaagc ttgttgactc
     3061 tactgataag gctgatcttc gtctcatcta ccttgctctc gctcacatga tcaagttccg
     3121 tggtcacttc cttatcgagg gtgaccttaa ccctgataac tccgacgtgg acaagctctt
     3181 catccagctc gtccagacct acaaccagct cttcgaggag aaccctatca acgcttccgg
     3241 tgtcgacgct aaggcgatcc tttccgctag gctctccaag tccaggcgtc tcgagaacct
     3301 catcgcccag ctccctggtg agaagaagaa cggtcttttc ggtaacctca tcgctctctc
     3361 cctcggtctg acccctaact tcaagtccaa cttcgacctc gctgaggacg ctaagcttca
     3421 gctctccaag gatacctacg acgatgatct cgacaacctc ctcgctcaga ttggagatca
     3481 gtacgctgat ctcttccttg ctgctaagaa cctctccgat gctatcctcc tttcggatat
     3541 ccttagggtt aacactgaga tcactaaggc tcctctttct gcttccatga tcaagcgcta
     3601 cgacgagcac caccaggacc tcaccctcct caaggctctt gttcgtcagc agctccccga
     3661 gaagtacaag gagatcttct tcgaccagtc caagaacggc tacgccggtt acattgacgg
     3721 tggagctagc caggaggagt tctacaagtt catcaagcca atccttgaga agatggatgg
     3781 tactgaggag cttctcgtta agcttaaccg tgaggacctc cttaggaagc agaggacttt
     3841 cgataacggc tctatccctc accagatcca ccttggtgag cttcacgcca tccttcgtag
     3901 gcaggaggac ttctaccctt tcctcaagga caaccgtgag aagatcgaga agatccttac
     3961 tttccgtatt ccttactacg ttggtcctct tgctcgtggt aactcccgtt tcgcttggat
     4021 gactaggaag tccgaggaga ctatcacccc ttggaacttc gaggaggttg ttgacaaggg
     4081 tgcttccgcc cagtccttca tcgagcgcat gaccaacttc gacaagaacc tccccaacga
     4141 gaaggtcctc cccaagcact ccctcctcta cgagtacttc acggtctaca acgagctcac
     4201 caaggtcaag tacgtcaccg agggtatgcg caagcctgcc ttcctctccg gcgagcagaa
     4261 gaaggctatc gttgacctcc tcttcaagac caaccgcaag gtcaccgtca agcagctcaa
     4321 ggaggactac ttcaagaaga tcgagtgctt cgactccgtc gagatcagcg gcgttgagga
     4381 ccgtttcaac gcttctctcg gtacctacca cgatctcctc aagatcatca aggacaagga
     4441 cttcctcgac aacgaggaga acgaggacat cctcgaggac atcgtcctca ctcttactct
     4501 cttcgaggat agggagatga tcgaggagag gctcaagact tacgctcatc tcttcgatga
     4561 caaggttatg aagcagctca agcgtcgccg ttacaccggt tggggtaggc tctcccgcaa
     4621 gctcatcaac ggtatcaggg ataagcagag cggcaagact atcctcgact tcctcaagtc
     4681 tgatggtttc gctaacagga acttcatgca gctcatccac gatgactctc ttaccttcaa
     4741 ggaggatatt cagaaggctc aggtgtccgg tcagggcgac tctctccacg agcacattgc
     4801 taaccttgct ggttcccctg ctatcaagaa gggcatcctt cagactgtta aggttgtcga
     4861 tgagcttgtc aaggttatgg gtcgtcacaa gcctgagaac atcgtcatcg agatggctcg
     4921 tgagaaccag actacccaga agggtcagaa gaactcgagg gagcgcatga agaggattga
     4981 ggagggtatc aaggagcttg gttctcagat ccttaaggag caccctgtcg agaacaccca
     5041 gctccagaac gagaagctct acctctacta cctccagaac ggtagggata tgtacgttga
     5101 ccaggagctc gacatcaaca ggctttctga ctacgacgtc gaccacattg ttcctcagtc
     5161 tttccttaag gatgactcca tcgacaacaa ggtcctcacg aggtccgaca agaacagggg
     5221 taagtcggac aacgtccctt ccgaggaggt tgtcaagaag atgaagaact actggaggca
     5281 gcttctcaac gctaagctca ttacccagag gaagttcgac aacctcacga aggctgagag
     5341 gggtggcctt tccgagcttg acaaggctgg tttcatcaag aggcagcttg ttgagacgag
     5401 gcagattacc aagcacgttg ctcagatcct cgattctagg atgaacacca agtacgacga
     5461 gaacgacaag ctcatccgcg aggtcaaggt gatcaccctc aagtccaagc tcgtctccga
     5521 cttccgcaag gacttccagt tctacaaggt ccgcgagatc aacaactacc accacgctca
     5581 cgatgcttac cttaacgctg tcgttggtac cgctcttatc aagaagtacc ctaagcttga
     5641 gtccgagttc gtctacggtg actacaaggt ctacgacgtt cgtaagatga tcgccaagtc
     5701 cgagcaggag atcggcaagg ccaccgccaa gtacttcttc tactccaaca tcatgaactt
     5761 cttcaagacc gagatcaccc tcgccaacgg cgagatccgc aagcgccctc ttatcgagac
     5821 gaacggtgag actggtgaga tcgtttggga caagggtcgc gacttcgcta ctgttcgcaa
     5881 ggtcctttct atgcctcagg ttaacatcgt caagaagacc gaggtccaga ccggtggctt
     5941 ctccaaggag tctatccttc caaagagaaa ctcggacaag ctcatcgcta ggaagaagga
     6001 ttgggaccct aagaagtacg gtggtttcga ctcccctact gtcgcctact ccgtcctcgt
     6061 ggtcgccaag gtggagaagg gtaagtcgaa gaagctcaag tccgtcaagg agctcctcgg
     6121 catcaccatc atggagcgct cctccttcga gaagaacccg atcgacttcc tcgaggccaa
     6181 gggctacaag gaggtcaaga aggacctcat catcaagctc cccaagtact ctcttttcga
     6241 gctcgagaac ggtcgtaaga ggatgctggc ttccgctggt gagctccaga agggtaacga
     6301 gcttgctctt ccttccaagt acgtgaactt cctctacctc gcctcccact acgagaagct
     6361 caagggttcc cctgaggata acgagcagaa gcagctcttc gtggagcagc acaagcacta
     6421 cctcgacgag atcatcgagc agatctccga gttctccaag cgcgtcatcc tcgctgacgc
     6481 taacctcgac aaggtcctct ccgcctacaa caagcaccgc gacaagccca tccgcgagca
     6541 ggccgagaac atcatccacc tcttcacgct cacgaacctc ggcgcccctg ctgctttcaa
     6601 gtacttcgac accaccatcg acaggaagcg ttacacgtcc accaaggagg ttctcgacgc
     6661 tactctcatc caccagtcca tcaccggtct ttacgagact cgtatcgacc tttcccagct
     6721 tggtggtgat aagcgtcctg ctgccaccaa aaaggccgga caggctaaga aaaagaagta
     6781 ggatccacct gatctagagc tagcgtttaa acaccggtga cgtccgatcg ttcaaacatt
     6841 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     6901 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     6961 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     7021 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     7081 gaattgatcc cccctcgaca gcttccggaa agggcgaatt cgcaactttg tatacaaaag
     7141 ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     7201 aacagactac ataatactgt aaaacacaac atatccagtc actatgccat ccagctgata
     7261 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     7321 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctc
//
