LOCUS       sequence-123955-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ147
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(99..890)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgacgcgtaa
       61 ctcacgttaa gggattttgg tcatgggtgg ctcgagggtt atttgccgac taccttggtg
      121 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
      181 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
      241 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
      301 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
      361 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
      421 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
      481 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
      541 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
      601 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
      661 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
      721 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
      781 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
      841 actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat aatgtttaac
      901 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
      961 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     1021 aaacagtcat aacaagccat gaaaaccgcc actgcg
//
