LOCUS       sequence-123963-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with pcoCas9 (Plant codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ150
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(12..135)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(180..432)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(443..490)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cagctggatg gcatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
       61 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt
      121 ttgtatacaa agttgcgaat tcgccctttc cggaagctgt cgagggggga tcaattcccg
      181 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
      241 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
      301 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
      361 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
      421 gtttgaacga tcggacgtct cacttcttct tcttagcctg tccagccttc ttggtagcag
      481 ctggtctctt atctcctcca agctgagaaa gatcgattct ggtctcgtaa agtccggtga
      541 tagactggtg gataagggta gcatcaagaa cctccttggt agaggtgtat ctttttctat
      601 cgatggtggt atcgaagtac ttgaaagcag ctggagcacc aaggttggta agggtgaaaa
      661 ggtggatgat gttctcagcc tgctctctga ttggcttatc tctgtgcttg ttgtaagcag
      721 aaagaacctt atcaaggtta gcatcagcaa ggataactct cttagagaac tcagagattt
      781 gctcgatgat ctcatcaagg tagtgcttgt gctgctcaac gaaaagctgc ttctgctcgt
      841 tatcctctgg agatcccttc aacttctcgt agtgagaagc aaggtaaagg aagttaacgt
      901 acttagatgg aagagcaagc tcgtttccct tctgaagctc tccagcagaa gcaagcattc
      961 tctttcttcc gttctcaagc tcgaaaagag agtactttgg caacttgatg ataagatcct
     1021 tcttaacctc cttgtatccc ttagcctcaa ggaaatcgat
//
