LOCUS       sequence-123964-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with pcoCas9 (Plant codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ150
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             112..159
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             166..186
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat ggattacaag
      121 gatgatgatg ataaggatta caaggatgat gatgataaga tggctccaaa gaagaagaga
      181 aaggttggaa tccacggagt tccagctgct gataagaagt actctatcgg acttgacatc
      241 ggaaccaact ctgttggatg ggctgttatc accgatgagt acaaggttcc atctaagaag
      301 ttcaaggttc ttggaaacac cgatagacac tctatcaaga agaaccttat cggtgctctt
      361 cttttcgatt ctggagagac cgctgaggct accagattga agagaaccgc tagaagaaga
      421 tacaccagaa gaaagaacag aatctgctac cttcaggaaa tcttctctaa cgagatggct
      481 aaggttgatg attctttctt ccacagactt gaggagtctt tccttgttga ggaggataag
      541 aagcacgaga gacacccaat cttcggaaac atcgttgatg aggttgctta ccacgagaag
      601 tacccaacca tctaccacct tagaaagaag ttggttgatt ctaccgataa ggctgatctt
      661 agacttatct accttgctct tgctcacatg atcaagttca gaggacactt ccttatcgag
      721 ggagacctta acccagataa ctctgatgtt gataagttgt tcatccagct tgttcagacc
      781 tacaaccagc ttttcgagga gaacccaatc aacgcttctg gagttgatgc taaggctatc
      841 ctttctgcta gactttctaa gtctcgtaga cttgagaacc ttatcgctca gcttccagga
      901 gagaagaaga acggactttt cggaaacctt atcgctcttt ctcttggact taccccaaac
      961 ttcaagtcta acttcgatct tgctgaggat gctaagttgc agctttctaa ggatacctac
     1021 gatgatgatc ttgataacct tcttgctcag atcgga
//
