LOCUS       sequence-123965-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 2nd gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ132A
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(352..371)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(353..371)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(376..392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(376..392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        481..784
                     /label=AtU6-1 promoter
                     /note="promoter for the Arabidopsis thaliana U6-1 snRNA 
                     gene (Waibel and Filipowicz, 1990)"
     misc_RNA        818..893
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat
       61 ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata
      121 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
      181 ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc
      241 ttgcggagaa ctgtgaatgc gcaaaccaac ccttgatcgg ggaagaacag tatgtcgagc
      301 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat cccctatagt
      361 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
      421 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagag gtctcccatg
      481 agaaatctca aaattccggc agaacaattt tgaatctcga tccgtagaaa ccagacggtc
      541 attgttttag ttccaccacg attatatttg aaatttacgt gagtgtgagt gagacttgca
      601 taagaaaata aaatctttag ttgggaaaaa attcaataat ataaatgggc ttgagaagga
      661 agcgagggat aggccttttt ctaaaatagg cccatttaag ctattaacaa tcttcaaaag
      721 taccacagcg cttaggtaaa gaaagcagct gagtttatat atggttagac acgaagtagt
      781 gattggagac gagatctagt ctgagtcgac cgtctctgtt ttagagctag aaatagcaag
      841 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
      901 ggcaaaaatt ttcagatttt ttcttcatct gtagatttct gggttttttt ttccgtttcg
      961 tgaatcataa gtgaagtttt ggatgcaaat ctgcgcgaaa aaagttggac ctgcaatgag
     1021 cttatttaga tagctaagac aaagtgattg gtccgttgtt tcagttctga ttgtcagaga
     1081 gtttgtttcg agtcggcgac
//
