LOCUS       sequence-123966-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 2nd gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ132A
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(482..557)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(591..894)
                     /label=AtU6-1 promoter
                     /note="promoter for the Arabidopsis thaliana U6-1 snRNA 
                     gene (Waibel and Filipowicz, 1990)"
     primer_bind     983..999
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     983..999
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1004..1023
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1004..1022
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgacgcgta
       61 actcacgtta agggattttg gtcatgggtg gctcgagggt ctcggtccgc tgatcctaaa
      121 tgctatcaag tttattatat ttttgtgaaa gaaatgttgt gtttcaatct ttggtctatc
      181 taatttcacc cttcataaga aaatgatgta gccgtcttga aacaaactct cgatacattt
      241 cttacccgaa atctggttaa caaaacgcat tggtgtcgcc gactcgaaac aaactctctg
      301 acaatcagaa ctgaaacaac ggaccaatca ctttgtctta gctatctaaa taagctcatt
      361 gcaggtccaa cttttttcgc gcagatttgc atccaaaact tcacttatga ttcacgaaac
      421 ggaaaaaaaa acccagaaat ctacagatga agaaaaaatc tgaaaatttt tgccaaaaaa
      481 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct
      541 atttctagct ctaaaacaga gacggtcgac tcagactaga tctcgtctcc aatcactact
      601 tcgtgtctaa ccatatataa actcagctgc tttctttacc taagcgctgt ggtacttttg
      661 aagattgtta atagcttaaa tgggcctatt ttagaaaaag gcctatccct cgcttccttc
      721 tcaagcccat ttatattatt gaattttttc ccaactaaag attttatttt cttatgcaag
      781 tctcactcac actcacgtaa atttcaaata taatcgtggt ggaactaaaa caatgaccgt
      841 ctggtttcta cggatcgaga ttcaaaattg ttctgccgga attttgagat ttctcatggg
      901 agacctctag aggaggcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
      961 ttgagacacg ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata
     1021 ggggatattt tattttctcc caatcaggct tgatccc
//
