LOCUS       sequence-123967-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter for Dux4 activation of RFPL4b transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  TITLE     Dux4 reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGL3 RFPL4b promoter
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      760..769
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(832..850)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cagaacattt ctctatcgat aggtacctct gttgtgactt ttactgctcc ttctgctctc
       61 gatacagaca ccataaaatt taccgttcat ccaagaacac ttttgagagt aaaaaatgga
      121 attattaata attacaggac aatagccttg cctagctgcc atctttctta tcctgtccag
      181 taagtacccc taagagtaga attgttgagt caaaggacat gaaaactgtt gtagatcctg
      241 tccgctcatg attctatcag gaataggaac accaatgatt acattagcca cgaggtgatt
      301 aggtttcagc agtgcatttc tgtatttgat ctccaacaca ttttgctttt taaagttgag
      361 ttctgaaatt agagacaaga gcagatggca gtagattcac tctaattcag ggagttatta
      421 agaaattttg aattttttta ctatataatt tgaagcctgc tttaatgtca tcatcataaa
      481 cggaagggtg ggttgatgag tttactcaga ttagagtggt acaacctttg gggcggagcc
      541 tctgactctg attggttgga caatgttttc caggcagtat aaaaacgctg gtgtctctcc
      601 gcggaaactc cgagaggagc agaggtctgt agaggtagag acgtaggctt cggatctttt
      661 agaattctgc tggaagtctc caaggagctc ttacgcgtgc tagcccgggc tcgagatctg
      721 cgatctaagt aagcttggca ttccggtact gttggtaaag ccaccatgga agacgccaaa
      781 aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa
      841 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
      901 catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa
      961 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
     1021 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg
     1081 aacgacattt
//
