LOCUS       sequence-123969-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 5th gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ135A
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(480..555)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(589..892)
                     /label=AtU6-1 promoter
                     /note="promoter for the Arabidopsis thaliana U6-1 snRNA 
                     gene (Waibel and Filipowicz, 1990)"
     primer_bind     981..997
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     981..997
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gacgcgtaac
       61 tcacgttaag ggattttggt catgggtggc tcgagggtct cctgcagctg atcctaaatg
      121 ctatcaagtt tattatattt ttgtgaaaga aatgttgtgt ttcaatcttt ggtctatcta
      181 atttcaccct tcataagaaa atgatgtagc cgtctggaaa caaactctcg atacatttct
      241 tacccgaaat ctggttaaca aaacgcattg gtgtcgccga ctcgaaacaa actctctgac
      301 aatcagaact gaaacaacgg accaatcact ttgtcttagc tatctaaata agctcattgc
      361 aggtccaact tttttcgcgc agatttgcat ccaaaacttc acttatgatt cacgaaacgg
      421 aaaaaaaaac ccagaaatct acagatgaag aaaaaatctg aaaatttttg ccaaaaaaag
      481 caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat
      541 ttctagctct aaaacagaga cggtcgactc agactagatc tcgtctccaa tcactacttc
      601 gtgtctaacc atatataaac tcagctgctt tctttaccta agcgctgtgg tacttttgaa
      661 gattgttaat agcttaaatg ggcctatttt agaaaaaggc ctatccctcg cttccttctc
      721 aagcccattt atattattga attttttccc aactaaagat tttattttct tatgcaagtc
      781 tcactcacac tcacgtaaat ttcaaatata atcgtggtgg aactaaaaca atgaccgtct
      841 ggtttctacg gatcgagatt caaaattgtt ctgccggaat tttgagattt ctaacaggag
      901 acctctagag gaggcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
      961 gagacacggg ccagagctgc caggaaacag ctatgaccat gtaatacgac tc
//
