LOCUS       sequence-123970-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector;7th gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ137A
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(351..370)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(352..370)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(375..391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(375..391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        480..783
                     /label=AtU6-1 promoter
                     /note="promoter for the Arabidopsis thaliana U6-1 snRNA 
                     gene (Waibel and Filipowicz, 1990)"
     misc_RNA        817..892
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt cacggcgatt
       61 tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
      121 cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
      181 gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
      241 tgcggagaac tgtgaatgcg caaaccaacc cttgatcggg gaagaacagt atgtcgagct
      301 attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatc ccctatagtg
      361 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
      421 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagagg tctcacggta
      481 gaaatctcaa aattccggca gaacaatttt gaatctcgat ccgtagaaac cagacggtca
      541 ttgttttagt tccaccacga ttatatttga aatttacgtg agtgtgagtg agacttgcat
      601 aagaaaataa aatctttagt tgggaaaaaa ttcaataata taaatgggct tgagaaggaa
      661 gcgagggata ggcctttttc taaaataggc ccatttaagc tattaacaat cttcaaaagt
      721 accacagcgc ttaggtaaag aaagcagctg agtttatata tggttagaca cgaagtagtg
      781 attggagacg agatctagtc tgagtcgacc gtctctgttt tagagctaga aatagcaagt
      841 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttg
      901 gcaaaaattt tcagattttt tcttcatctg tagatttctg ggtttttttt tccgtttcgt
      961 gaatcataag tgaagttttg gatgcaaatc tgcgcgaaaa aagttggacc tgcaatgagc
     1021 tta
//
