LOCUS       sequence-123973-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 8th gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ138A
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(354..373)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(355..373)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(378..394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(378..394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        483..786
                     /label=AtU6-1 promoter
                     /note="promoter for the Arabidopsis thaliana U6-1 snRNA 
                     gene (Waibel and Filipowicz, 1990)"
     misc_RNA        820..895
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg
       61 atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta
      121 taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga
      181 atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
      241 tcttgcggag aactgtgaat gcgcaaacca acccttgatc ggggaagaac agtatgtcga
      301 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat atcccctata
      361 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
      421 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag aggtctccga
      481 aaagaaatct caaaattccg gcagaacaat tttgaatctc gatccgtaga aaccagacgg
      541 tcattgtttt agttccacca cgattatatt tgaaatttac gtgagtgtga gtgagacttg
      601 cataagaaaa taaaatcttt agttgggaaa aaattcaata atataaatgg gcttgagaag
      661 gaagcgaggg ataggccttt ttctaaaata ggcccattta agctattaac aatcttcaaa
      721 agtaccacag cgcttaggta aagaaagcag ctgagtttat atatggttag acacgaagta
      781 gtgattggag acgagatcta gtctgagtcg accgtctctg ttttagagct agaaatagca
      841 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      901 ttggcaaaaa ttttcagatt ttttcttcat ctgtagattt ctgggttttt ttttccgttt
      961 cgtgaatcat aagtgaagtt ttggatgcaa atctgcgcga aaaaagttgg acctgcaatg
//
