LOCUS       sequence-123979-        1137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase in lentivirus backbone to report Dux4 activation of 
            RFPL4b promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1137)
  TITLE     Dux4 reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRRLSIN.cPPT.RFPL4b.Luciferase.WPRE
FEATURES             Location/Qualifiers
     source          1..1137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      812..821
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(884..902)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact
       61 agcctcgaga agcttgatct ctgttgtgac ttttactgct ccttctgctc tcgatacaga
      121 caccataaaa tttaccgttc atccaagaac acttttgaga gtaaaaaatg gaattattaa
      181 taattacagg acaatagcct tgcctagctg ccatctttct tatcctgtcc agtaagtacc
      241 cctaagagta gaattgttga gtcaaaggac atgaaaactg ttgtagatcc tgtccgctca
      301 tgattctatc aggaatagga acaccaatga ttacattagc cacgaggtga ttaggtttca
      361 gcagtgcatt tctgtatttg atctccaaca cattttgctt tttaaagttg agttctgaaa
      421 ttagagacaa gagcagatgg cagtagattc actctaattc agggagttat taagaaattt
      481 tgaatttttt tactatataa tttgaagcct gctttaatgt catcatcata aacggaaggg
      541 tgggttgatg agtttactca gattagagtg gtacaacctt tggggcggag cctctgactc
      601 tgattggttg gacaatgttt tccaggcagt ataaaaacgc tggtgtctct ccgcggaaac
      661 tccgagagga gcagaggtct gtagaggtag agacgtaggc ttcggatctt ttagaattct
      721 gctggaagtc tccaaggagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa
      781 gtaagcttgg cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa
      841 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
      901 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
      961 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
     1021 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     1081 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacga
//
