LOCUS       sequence-123982-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 2nd gRNA under AtU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ132B
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(288..363)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     811..827
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     811..827
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     832..851
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        832..850
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
       61 cacgttaagg gattttggtc atgggtggct cgagggtctc ggtcccacaa tgttttaatt
      121 taggctcatt atagattcta gttccaaaat tcaagaatta tctctaaatc agtcatgaaa
      181 gacattcaca tggatgttgg tttgttctag gttttcaata tctacaatga tcgatataca
      241 aatttaagtt tatggcaaaa aaaagaaaaa aggaaaggga aaaaaaagca ccgactcggt
      301 gccacttttt caagttgata acggactagc cttattttaa cttgctattt ctagctctaa
      361 aacagagacg gtcgactcag actagatctc gtctctgacc aatggtgctc cctcagtgtt
      421 atatatacaa caagatttgt ttagatttga gcgatgtggg agaaattagg agcttatatg
      481 aaacgcatac atgggtactg ggcatgggta ttagtgaata tatggacgat agcccattaa
      541 attacataaa aagcccactt atcttattgc tcgatataag ctccgtatat aaactgaagt
      601 tgatcctaaa agttcacttg tttcgtccaa catagccaaa acagatgttc ttaaacccag
      661 acgccatttg tttgattcag ttatccatca caggctgagc cataagaaaa atttaaagta
      721 aacatgggag acctctagag gaggcatgat gatatatttt tatcttgtgc aatgtaacat
      781 cagagatttt gagacacggg ccagagctgc caggaaacag ctatgaccat gtaatacgac
      841 tcactatagg ggatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
      901 tagctcgaca tactgttctt ccccgatcaa gggttggttt gcgcattcac agttctccgc
      961 aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt
     1021 ccattcaggt cgaggtggcc cggctccatg c
//
