LOCUS       sequence-123986-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone.
            This vector is used for cloning a specific sgRNA by BamHI, to be 
            co-expressed with Cas9 for genome editing in Trypanosoma cruzi..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Lander N, Li ZH, Niyogi S, Docampo R
  TITLE     CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 
            Genes in Trypanosoma cruzi Reveals Their Role in Flagellar 
            Attachment.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15.
  PUBMED    26199333
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e01012-15. doi"; pages: " 
            10.1128/mBio.01012-15"
COMMENT     Sequence Label: Cas9/pTREX-n
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctgtggaag aagctgtcgt ccaccttggc catctcgttg ctgaagatct cttgcagata
       61 gcagatccgg ttcttccgtc tggtgtatct tcttctggcg gttctcttca gccgggtggc
      121 ctcggctgtt tctccgctgt cgaacagcag ggcgccgatc aggttcttct tgatgctgtg
      181 ccggtcggtg ttgcccagca ccttgaattt cttgctgggc accttgtact cgtcggtgat
      241 cacggcccag cccacagagt tggtgccgat gtccaggccg atgctgtact tcttgtccat
      301 tctagacaag acaacttata gagcaaaaaa aaaaaagaaa agacatcata aaagaagttg
      361 gaaaagaaaa aaggtaaaaa caatttaaca caacaataat aagtggataa aacaacaaag
      421 aaaaagtatt ataataacaa tcataataat ttcgctttcg tgcgtgaaaa tgtttcttag
      481 tgaatccgct gcatattttg aagaaactcg ttgggggatc cactagaact cttgcacaga
      541 cttggcatcc acacggcgct ttgactcccc ttctgaaaaa aaaagaacac gttgattccc
      601 cttttgattt cccacgaatg cgaagcacaa ttcacgcaca gccaacggca gggagagacg
      661 ataaggtaaa atacacacaa gtggtggaat cacaaaagga cacgcacagc acgcaaataa
      721 acattgcaca cggcatgaca ttttgcgacc cacacacaat gtgtacacta caattatatg
      781 aaaatccctc ccccattccg cgggtgcgcc gcaaagggcc aaaa
//
