LOCUS       sequence-123988-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone.
            This vector is used for cloning a specific sgRNA by BamHI, to be 
            co-expressed with Cas9 for genome editing in Trypanosoma cruzi..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Lander N, Li ZH, Niyogi S, Docampo R
  TITLE     CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 
            Genes in Trypanosoma cruzi Reveals Their Role in Flagellar 
            Attachment.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15.
  PUBMED    26199333
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e01012-15. doi"; pages: " 
            10.1128/mBio.01012-15"
COMMENT     Sequence Label: Cas9/pTREX-n
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..43)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(50..70)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(83..109)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(89..115)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgctcccact accaatgccg tccactttcc gctttttctt aggatcttcc accttgcgtt
       61 ttttcttggg ggagccgctg cccaggctgg cataatcggg cacgtcatag ggataggcgt
      121 cgcctcccag ctgagacagg tcgatccgtg tctcgtacag gccggtgatg ctctggtgga
      181 tcagggtggc gtccagcacc tctttggtgc tggtgtacct cttccggtcg atggtggtgt
      241 caaagtactt gaaggcggca ggggctccca gattggtcag ggtaaacagg tggatgatat
      301 tctcggcctg ctctctgata ggcttgtctc tgtgcttgtt gtaggcgctc agcaccttgt
      361 ccagattagc gtcggccagg atcactctct tggagaactc gctgatctgc tcgatgatct
      421 cgtccaggta gtgtttgtgc tgttccacaa acagctgttt ctgctcatta tcctcggggg
      481 agcccttcag cttctcatag tggctggcca ggtacaggaa gttcacatat ttggagggca
      541 gggccagttc gtttcccttc tgcagttcgc cggcagaggc cagcattctc ttccggccgt
      601 tttccagctc gaacagggag tacttaggca gcttgatgat caggtccttt ttcacttctt
      661 tgtagccctt ggcttccaga aagtcgatgg gattcttctc gaagctgctt ctttccatga
      721 tggtgatccc cagcagctct ttcacactct tcagtttctt ggacttgccc ttttccactt
      781 tggccaccac cagcacagaa taggccacgg tggggctgtc gaagccgccg tacttcttag
      841 ggtcccagtc cttctttctg gcgatcagct tgtcgctgtt cctcttgggc aggatagact
      901 ct
//
